Untargeted lipidomics
Get a semi quantitative profiling of many classes of biologically relevant lipids using our high resolution instruments.
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Get a semi quantitative profiling of many classes of biologically relevant lipids using our high resolution instruments.
The high resolution and MS/MS all technology of our Sciex instruments enables for fast and reliable lipidomics studies without requiring chromatographic separation of the lipids prior to mass spectrometry analysis. In such an experiment, the mass spectrometer records everything that is in the sample in a data independent manner. This means that you do not have to know beforehand what is in your sample to get a quantification value for it. Using such a workflow, we routinely get quantitative data for several thousand lipids in one experiment. Untargeted lipidomics are generally used to discover trends in samples and identify lipids of interest, which can be further analyzed in a targeted LC-MS/MS workflow later on.
Although infusion lipidomics can answer many of the needs associated with sample lipid profiling, some workflows do require chromatographic separation before MS analysis. For example, the quantification of isobaric lipids can only be done if the lipids are somehow separated before entering the mass spectrometer. In these cases, we would choose a column that is suitable for the separation of your lipids of interest and still be able to perform large spectrum lipidomics profiling experiments.
Non-exhaustive list of lipid classes covered by these methods
Reports for untargeted lipidomics experiment will be customized to your needs. We typically report both individual lipids and the sum of all the lipids in classes of interest. Advanced statistics (PCA, heatmap clustering) can be included if needed. In any cases, a quick chat with our experts can be scheduled to ensure that you get the results that you are looking for.