N-Terminal Protein Sequencing

Chemistry hand in hand with mass spec.

Using a combination of chemical modifications, enrichment methods, and mass spectrometry, we can produce a map of the “N-Terminome” of a sample.

Allumiqs Capabilities

Why study the N-Terminal of a protein?

Many precursor proteins must undergo post-translational modifications to become biologically active. For example, the majority of peptide hormones are produced as pro-hormones that need to be proteolytically cleaved for hormonal activity. Therefore, workflows that enable the identification of the N-Terminal of one or more proteins can become very interesting. Using a combination of chemical modifications, enrichment methods, and mass spectrometry, we can produce a map of the “N-Terminome” of a sample.

N-Terminal Mapping

The workflow for studying the N-Terminus of proteins

The workflow outlined here can be used to study the N-Terminus of pure proteins or to identify variations in the N-Termini of every detectable protein in a sample.

Get started with your study