MagReSyn Streptavidin

High capacity for capture of biotinylated molecules

MagReSyn^® Streptavidin microparticles contain covalently coupled streptavidin. The porous nature of the polymer technology allows for exceptional streptavidin capacity, translating into increased power for the immobilization/capture of biotinylated biomolecules. The high streptavidin load allows the use of as little as 10 µl beads, improving experimental miniaturization for high-throughput automated analysis. Molecular applications of MagReSyn^® Streptavidin microparticles include, protein immobilization and purification; nucleic acid capture assays and preparation of single-stranded DNA templates for e.g. SELEX and pyrosequencing. The immobilization of biotinylated antibodies may subsequently be used in e.g. immunoassay.

• Support: Proprietary polymer microparticles containing iron oxide (magnetite) with immobilised Streptavidin
• Binding capacity (DNA): > 30 000 pmoles.ml^-1 (Biotinylated oligo, 25-mer)
• Binding capacity (Protein): > 3 mg.ml^-1 (Biotinylated IgG)
• Bead size: ~5-10 µm average
• Formulation: 10 mg.ml^-1 suspension in sodium phosphate buffer