Label-free quantitative proteomics services

Explore the power of data independent acquisition (DIA), a.k.a. SWATH. Get quantitative data for up to 6500 proteins in your samples with our label-free quantitative proteomics workflow*. 

Screenshot of a SWATH analysis

The very best in label-free proteomic quantification

Our team of scientist spent the best of the last few years perfecting our label-free quantitative proteomics service. By using a technology called SWATHTM, or data independent acquisition (DIA), we can record and quantify every single ionizable peptide in your sample. This type of analysis if perfect to compare the proteomes of cells or tissues between two or more treatments.

Divide to see better

The SWATHTM technology uses a clever way to record everything while maintaining the ability to easily search the results after the analysis. The key to this acquisition method is a fragmentation of the studied mass range in smaller windows. Every peptide that falls into one window is fragmented and the machine records its MS/MS signature. Then, by using an ion library, we are able to deconvolute the signal and extract quantitative information for every peptide, at the MS/MS level. 

For more information about SWATHTM, you can refer to blog posts and videos that we produced on the subject.

SWATH Workflow

Data report

The data for label-free quantitative proteomics experiments will be delivered in a spreadsheet. We will provide basic statistics such as average, standard deviation and %CV and fold change for group comparisons. Depending on the number of replicates, we will generate either a confidence interval or a Student's T test to assess significance. In addition, we will provide an interactive data tab where you can visualize relevant information for a given protein. If needed, we can also generate more advanced statistical analyses, such as a Principal Component Analysis (PCA), heatmap clustering and gene ontology. For more information, please refer to our blog post on the matter.

Blog posts

Quantitative proteomics using SWATH: What is it and how it works?

Q&A about SWATH

What you should expect from your proteomics experiments


Quantitative SWATH proteomics, why is it better?

*On specific workflows. Certain conditions apply.

Contact us for your needs in quantitative proteomics

My personal experience of collaborating with PhenoSwitch Bioscience was really amazing and great. Ribosomal protein paralogs have very limited number of amino acid differences. If it were not for PhenoSwitch, it would not have been possible to get such a high quality identification and quantification of duplicated ribosomal proteins in yeast. I am highly indebted and thankful for their perseverance, commitment, help and guidance during the tough times of optimization. If you you need to use MS for identification and quantification of your molecules, I strongly recommend you to talk to these guys!
Mustafa Malik Ghulam, Post Doctoral Fellow, RNA Therapeutic Institute, Umass Medical School, Worcester, Massachussetts

"PhenoSwitch Bioscience Inc has provided us with outstanding efficacious service, high quality data and exemplary data analysis over the past three years."

Stephen Naylor, Ph.D., CEO at ReNeuroGen LCC, Milwaukee, USA
"They have provided excellent data in a timely manner on a number of metabolism and protein studies ongoing in my group"
Klaus Klarskov, Ph.D., Professor at Université de Sherbrooke, Canada
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