Label-free quantitative proteomics services
Explore the power of data independent acquisition (DIA), a.k.a. SWATH. Get quantitative data for up to 6500 proteins in your samples with our label-free quantitative proteomics workflow*.
EN
Explore the power of data independent acquisition (DIA), a.k.a. SWATH. Get quantitative data for up to 6500 proteins in your samples with our label-free quantitative proteomics workflow*.
Our team of scientist spent the best of the last few years perfecting our label-free quantitative proteomics service. By using a technology called SWATHTM, or data independent acquisition (DIA), we can record and quantify every single ionizable peptide in your sample. This type of analysis if perfect to compare the proteomes of cells or tissues between two or more treatments.
Divide to see better
The SWATHTM technology uses a clever way to record everything while maintaining the ability to easily search the results after the analysis. The key to this acquisition method is a fragmentation of the studied mass range in smaller windows. Every peptide that falls into one window is fragmented and the machine records its MS/MS signature. Then, by using an ion library, we are able to deconvolute the signal and extract quantitative information for every peptide, at the MS/MS level.
For more information about SWATHTM, you can refer to blog posts and videos that we produced on the subject.
The data for label-free quantitative proteomics experiments will be delivered in a spreadsheet. We will provide basic statistics such as average, standard deviation and %CV and fold change for group comparisons. Depending on the number of replicates, we will generate either a confidence interval or a Student's T test to assess significance. In addition, we will provide an interactive data tab where you can visualize relevant information for a given protein. If needed, we can also generate more advanced statistical analyses, such as a Principal Component Analysis (PCA), heatmap clustering and gene ontology. For more information, please refer to our blog post on the matter.
Blog posts
Quantitative proteomics using SWATH: What is it and how it works?
What you should expect from your proteomics experiments
Video
Quantitative SWATH proteomics, why is it better?