Drug target identification

Modify, crosslink and identify

Identifying the target of a drug can seem to be a hard process. And it is. However, using a clever combination of medicinal chemistry, well-designed protocol and mass spectrometry greatly enhances your chances of success. Here's how we proceed:

1. Assist in the synthesis of your drug with reactive groups (if needed);
2. Design the experiment based on your insights;
3. Perform the experiment in 3 steps:
1. Incubate the drug and crosslink;
2. Purify the drug/target complex from the protein solution;
3. Analyze the complex using LC-MS/MS and identify the target.

As a side note, using a quantitative proteomics workflow enables to get clearer insights in the real target of the drug, as it is compatible with dose-response experiments. This is why we design the protocols in accordance with our label-free quantitative proteomics workflow.

Data report

Provided information for drug-target identification data reports includes the raw and analyzed data as well as a list of the most probable targets of the studied drug. Additionally, an in-depth explanation on what qualifies the proteins in the list as potential targets will be offered.